TATA-binding protein is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably only one of the proteins involved in positioning RNA polymerase II.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the middle region of human TATA binding protein (EEQSRLAARKYARVVQKLGF) was used as the immunogen for this TBP antibody (100% homologous in human, mouse and rat).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
P20226
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the TBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.