Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988) Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)
Host
Rabbit
Immunogen
Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
Involvement In Disease
Osteogenesis imperfecta 17 (OI17)
Raised In
Rabbit
Reactivity
Human
Regulatory
RUO
Relevance
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990) Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988) Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of total SPARC protein.
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Protein Families
SPARC family
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.