activator of RNA decay; ARD-1; ARD1; EC 3.1.4.-; NIPP1
Antigen
PPP1R8
Categories
Primary Antibodies
Clonality
polyclonal
Description
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform doesnot inhibit PP-1. May be implicated in mRNA splicing.Wang M., Proc. Natl. Acad. Sci. U.S.A. 91:10591-10595(1994).Van Eynde A., Eur. J. Biochem. 261:291-300(1999).Liu J.P., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Host
Rabbit
Immunogen
Synthesized peptide derived from internal of Human PPP1R8.
Raised In
Rabbit
Reactivity
Human, Mouse
Regulatory
RUO
Relevance
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform doesnot inhibit PP-1. May be implicated in mRNA splicing.
Wang M., Proc. Natl. Acad. Sci. U.S.A. 91:10591-10595(1994). Van Eynde A., Eur. J. Biochem. 261:291-300(1999). Liu J.P., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total PPP1R8 protein.
Subcellular Location
Nucleus, Nucleus speckle, Note=Primarily, but not exclusively, nuclear, SUBCELLULAR LOCATION: Isoform Gamma: Cytoplasm
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.; FUNCTION
Tissue Specificity
Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform
Weight
28kDa
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.