JV18-1; MADH2; Mad-related protein 2; Mothers against DPP homolog 2;
Antigen
Smad2
Categories
Primary Antibodies ; Phospho Antibodies
Clonality
polyclonal
Description
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagu篓 J (1999)Genes Dev 13, 804-16 .
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine250(E-L-S(p)-P-T) derived from Human Smad2 .
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, MassaguJ (1999)Genes Dev 13, 804-16 .
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.