Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

Mfr.No.	AF3810
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232., A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232.
Isotype	IgG
Reactivity	Human, Mouse, Rat, Monkey

Rgd	AB_2847124
Uniprot	Q15418/P51812/Q15349/Q9UK32
Gene Id	6196,6197,6195,27330
Gene Name	RPS6KA2,RPS6KA3,RPS6KA1,RPS6KA6

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	WB, IHC, ELISA
Dilution	IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

Western blot analysis of extracts from various samples, using Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody. Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides, Lane 2: Hela cells(serum starvation treatment), Lane 3: VERO cells(serum starvation treatment).Western blot analysis of extracts from various samples, using Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody.

AF3810 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3810 at 1/100 staining Mouse stomach tissue by IHC-P.

AF3810 at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3810 at 1/100 staining Rat skin tissue by IHC-P.

AF3810 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3810 at 1/100 staining Rat testis tissue by IHC-P.

AF3810 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3810 at 1/100 staining Rat stomach tissue by IHC-P.

AF3810 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3810 at 1/100 staining Rat brain tissue by IHC-P.