Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.English J.M., Vanderbilt C.A., Xu S., Marcus S., Cobb M.H.J. Biol. Chem. 270:28897-28902(1995)
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
English J.M., Vanderbilt C.A., Xu S., Marcus S., Cobb M.H.J. Biol. Chem. 270:28897-28902(1995)
Species
Rattus Norvegicus (Rat)
Specificity
The antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.
Subcellular Location
Isoform Beta: Cytoplasm, cytosol, SUBCELLULAR LOCATION: Isoform Alpha-1: Membrane, Note=The alternatively spliced exon in alpha isoform resemble conserved sequences that mediate interactions with the cytoskeleton, thereby explaining the differential localization, SUBCELLULAR LOCATION: Isoform Alpha-2: Membrane
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis (By similarity).
Pathway
MAPK signaling pathway Gap junction Neurotrophin signaling pathway
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
MEK5 beta is ubiquitously distributed with highest levels in the liver, whereas MEK5 alpha is expressed only in liver and brain.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB
Description
ELISA ; WB, WB ; ELISA
Dilution
WB
1:500-1:1000
Tested Application
ELISA,WB;WB:1:500-1:1000
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Reviews of Phospho-Map2k5 (Ser311/Thr315) Antibody