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Home / Primary Antibodies

Phospho-FGFR1/FGFR2 (Tyr463/466) Antibody

SKU : BHA10560208

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA443701
Alternative Names	BFGFR; CEK; FLG; CD331; FLT2
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Itoh N., Biochem. Biophys. Res. Commun. 169:680-685(1990).Dionne C.A., EMBO J. 9:2685-2692(1990).Johnson D.E., Mol. Cell. Biol. 10:4728-4736(1990).
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 463/466(S-E-Y(p)-E-L) derived from Human FGFR1/2.
Involvement In Disease	Pfeiffer syndrome (PS); Hypogonadotropic hypogonadism 2 with or without anosmia (HH2); Osteoglophonic dysplasia (OGD); Hartsfield syndrome (HRTFDS); Trigonocephaly 1 (TRIGNO1); Encephalocraniocutaneous lipomatosis (ECCL); Jackson-Weiss syndrome (JWS)
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Itoh N., Biochem. Biophys. Res. Commun. 169:680-685(1990). Dionne C.A., EMBO J. 9:2685-2692(1990). Johnson D.E., Mol. Cell. Biol. 10:4728-4736(1990).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of FGFR1/2 only when phosphorylated at tyrosine 463/466.
Subcellular Location	Cell membrane, Single-pass type I membrane protein, Nucleus, Cytoplasm, cytosol, Cytoplasmic vesicle

Uniprot	P11362
Database Links	HGNC:3688 OMIM:101600 KEGG:hsa:2260 STRING:9606.ENSP00000393312 UniGene:Hs.264887
Function	Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Pathway	MAPK signaling pathway PI3K-Akt signaling pathway Regulation of actin cytoskeleton Adherens junction Ras signaling pathway Signaling pathways regulating pluripotency of stem cells Rap1 signaling pathway
Protein Families	Protein kinase superfamily, Tyr protein kinase family, Fibroblast growth factor receptor subfamily
Tissue Specificity	Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560208	CSB-PA443701	100ul	$360		Add to Cart

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