Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P12004
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml