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Home / Primary Antibodies

MBD3 Antibody (V9207)

SKU : BHA17119596

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NSJ Bioreagents

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Mfr.No.	V9207, V9207-20UG, V9207-100UG
Clonality	Monoclonal (mouse origin)
Description	Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5 they include methyl-CpG binding protein 1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. MBD1 binds in a context specific manner to methyl-CpG rich domains and, in tu, mediates the transcriptional inhibition that is commonly observed with DNA methylation. Similarly, MBD2 inhibits transcription of methylated genes by associating with histone deacetylase (HDAC1) within the MeCP1 repressor complex. In addition, MBD4, which is also designated MED1, associates with the mismatch repair protein MLH1 and preferentially binds to methylated cytosine residues in mismatched base pairs. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Recombinant full-length human MBD3 protein was used as the immunogen for the MBD3 antibody.
Isotype	Mouse IgG2b
Species Reactivity	Human
Note	Optimal dilution of the MBD3 antibody should be determined by the researcher.

Uniprot	O95983

Format	Purified
Purity	Protein A/G affinity
Storage	Aliquot the MBD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Applications	FACS, IF
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml

Product Manual	Datasheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17119596-1	V9207-20UG	20ug	$219		Add to Cart
BHA17119596-2	V9207-100UG	100ug	$499		Add to Cart

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