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Home / Antibodies

KRT7 Antibody / Cytokeratin 7 (V2661SAF)

SKU : BHA17124138

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NSJ Bioreagents

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Mfr.No.	V2661SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant full-length human KRT7 protein was used as the immunogen for the Keratin 7 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Keratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P08729

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Keratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	FACS, IF, IHC-P
Description	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124138	V2661SAF-100UG	100 ug	$499		Add to Cart

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