Heat shock transcription factor 2 is a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Mouse HSF2 was expressed in all three embryonic layers at embryonic day 7.5 and the head fold was strongly stained at embryonic day 8.5. In adults, expression is detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells. It mediates Hsp70i bookmarking, binding to this promoter in mitotic cells, recruits protein phosphatase-2A, and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. And HSF2-null mice were born at the expected mendelian ratio but had brain abnormalities and meiotic and gametogenic defects in both genders.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the middle region of human HSF2 (KQERDGPVEFQHPYFKQGQDD) was used as the immunogen for this HSF2 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
Q03933
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the HSF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IF, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells