Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the Cytokeratin 18/Keratin 18 protein is coded for by 7 exons. By Southern blotting using the genomic DNA PCR product, the gene for Cytokeratin 18 has been assigned to chromosome 12. Mutation of human Keratin 18 has been associated with cryptogenic cirrhosis.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines was used as the immunogen for this Cytokeratin 18 antibody.
Isotype
IgG1
Predicted Reactivity
Human, rat
Reactivity
rat, Human
Recombinant
No
Gene Id
3875
Clone No
CY-90
Format
Ascites
Purification
Unpurified ascites
Storage
After reconstitution, the Cytokeratin 18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IHC-F
Dilution
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 0.5-2ug/ml; formalin or acetone fixed tissues