Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Purified CD44 antigen (PGp-1) from lymphocyte membrane was used as the immunogen for the CD44 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse
Note
Optimal dilution of the CD44 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P16070
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IF, IHC-P
Description
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT