Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
The stated application concentrations are suggested starting points. Titration of the Calnexin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Uniprot
P27824
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Calnexin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IF/ICC, IHC-P
Description
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT