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Home / Antibodies

BRCA1 (Ab-1524) Antibody

SKU : BHA10558994

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA904513
Alternative Names	RNF53
Categories	Primary Antibodies
Clonality	polyclonal
Description	The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylationLin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
Host	Rabbit
Immunogen	Peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from Human BRCA1.
Involvement In Disease	Breast cancer (BC); Breast-ovarian cancer, familial, 1 (BROVCA1); Ovarian cancer (OC); Pancreatic cancer 4 (PNCA4)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489 Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718 Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135 Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13 Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total BRCA1 protein.
Subcellular Location	Nucleus, Chromosome, Cytoplasm

Uniprot	P38398
Database Links	HGNC:1100 OMIM:113705 KEGG:hsa:672 STRING:9606.ENSP00000418960 UniGene:Hs.194143
Function	E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator
Pathway	PI3K-Akt signaling pathway Ubiquitin mediated proteolysis
Tissue Specificity	Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:1000
IF	1:100-1:200

Tested Application

ELISA,WB,IF;WB:1:500-1:1000,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558994	CSB-PA904513	100ul	$297		Add to Cart

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