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Home / Antibodies

Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) Antibody

SKU : BHA10922926

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y051648
Categories	Primary Antibodies
Clonality	polyclonal
Description	The antibody is a rabbit antibody to the regulatory subunit Ib of protein kinase-A, made with a synthetic peptide based on the rat sequence. The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as as cryoprotectant.Protein KinaseA is a critical kinase involved in numerous biological functions. The regulatory subunits of PKA allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue-specific manner. RP1PKARIb works well for Western Blotting applications, and when used against the reduced protein identifies bands at 55 kD and 53 kD, as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. RP1PKARIb recognized human, rat, mouse and rabbit PKARIb. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Formulation	Liquid
Host	Rabbit(Rb)
Guarantee	abm guarantees that all our Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	Synthetic peptide of Rat Protein Kinase A regulatory subunit I beta.
Isotype	IgG
Quantity	100μg
Recommended Dilution	Recommended dilutionsWB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa. This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Relevance	Protein Kinase A is a critical kinase involved in numerous biological functions. The regulatory subunits of PKA allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue specific manner. The type 1(RI) and type 2 (RII) subunit of cyclic AMP dependent protein kinase A (PKA) is a polyfunctional protein that is present in most tissues and cells.
Shipping Condition	Ice Packs
Species Reactivity	Human ; Mouse ; Rat
Specificity	The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb).

Gene Name	Pka
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Immunogen Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Condition	-20C

Description	WB

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SKU	Supplier No.	Size	Your price	Quantity
BHA10922926	Y051648	100 ug	$1357.50		Add to Cart

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