Mouse trypsin ELISA Kit

Mouse trypsin ELISA Kit

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Mfr.No.

CSB-E09900m

Alternative Names

trypsin

Reactivity

Mouse

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	87	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	91-102
1:4	Average %	90
	Range %	85-94
1:8	Average %	85
	Range %	80-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.374	2.452	2.413	2.313
500	1.619	1.676	1.648	1.548
250	0.936	1.004	0.970	0.870
125	0.537	0.549	0.543	0.443
62.5	0.381	0.374	0.378	0.278
31.2	0.257	0.259	0.258	0.158
15.6	0.168	0.164	0.166	0.066
0	0.102	0.098	0.100

Species

Mus musculus

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

Antigen	Trypsin

Applications

ELISA

Research Area

trypsin

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Antitumor Activity of Tenacissoside H on Esophageal Cancer through Arresting Cell Cycle and Regulating PI3K/Akt-NF-kB Transduction Cascade. Jia YS.et al,Evid Based Complement Alternat Med.,501: 464937.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.