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Home / ELISA Kit

Mouse Protein Wnt-11 (WNT11) ELISA Kit

SKU : BHE13705172

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT16 is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.

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Mfr.No.	KTE70010
Description	This Mouse Protein Wnt-11 (WNT11) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT11.
Alternative Names	WNT11; HWNT11; MGC141946; MGC141948;
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Protein Wnt-11 microplate Mouse Protein Wnt-11 standard Mouse Protein Wnt-11 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Protein Wnt-11 (WNT11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse WNT11. No significant cross-reactivity or interference between Mouse WNT11 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT16 is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.
Notes	This Mouse Protein Wnt-11 (WNT11) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT11 in samples. An antibody specific for WNT11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	22411
Antigen	WNT11
Tag	WNT11
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Protein Wnt-11 (WNT11) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT11 in samples. An antibody specific for WNT11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705172	KTE70010	48 T	$339		Add to Cart
BHE13705172	KTE70010	96 T	$589		Add to Cart
BHE13705172	KTE70010	96 Tx5	$2629		Add to Cart
BHE13705172	KTE70010	96 Tx50	$24569		Add to Cart

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