Matrices listed below were spiked with certain level of PKCalpha and the recovery rates were calculated by comparing the measured value to the expected amount of PKCalpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
95
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCalpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PRKCA(Protein kinase C alpha type)/PKCA/AAG6/PKC-A/PKC-alpha/PRKACA/aging-associated gene 6/protein kinase C, alpha
Notes
For Research Use Only
UniProt ID
P20444
Antigen
PKCα
Applications
ELISA
Applications Notes
PKCalpha ELISA Kit allows for the in vitro quantitative determination of PKCalpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse PKCalpha(Protein kinase C alpha type) ELISA Kit