Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

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Mfr.No.

CSB-E11040m

Alternative Names

Mucin-5 subtype AC,MUC5AC

Reactivity

Mouse

Detection Range

4.688 ng/mL-300 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	98	92-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-105
1:2	Average %	93
	Range %	89-99
1:4	Average %	95
	Range %	89-101
1:8	Average %	95
	Range %	83-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?IU/ml	OD1	OD2	Average
0	1.800	1.864	1.832
2	1.569	1.581	1.575
8	1.304	1.327	1.316
32	0.812	0.805	0.809
100	0.389	0.393	0.391
400	0.180	0.183	0.182

Species

Mus musculus

Sensitivity

1.172 ng/mL

Limit Of Detection

450 nm

Antigen	Mucin-5 subtype AC,MUC5AC

Applications

ELISA

Research Area

MUC5AC

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice /,PLOS ONE,2012 7(7):e41088. A Dissociated Glucocorticoid Receptor Modulator Reduces Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma /,The Journal of Immunology,2012 Apr 1;188(7):3478-87. The immune profile associated with acute allergic asthma accelerates clearance of influenza virus Samarasinghe AE et al,Immunol Cell Biol,Jan 28. Chronic intermittent hypoxiaDecreases pulmonary clearance of 99mTc-labelled particulate matter in mice Fu C.et al,Am J Transl Res,Jun 15

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SKU	Supplier No.	Size	Your price
BHE10506275	CSB-E11040m	96T	$695	Add to Cart
BHE10506275	CSB-E11040m	5x96T	$3336	Add to Cart
BHE10506275	CSB-E11040m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.