Mouse Kidney injury molecule 1,Kim-1 ELISA Kit

Mouse Kidney injury molecule 1,Kim-1 ELISA Kit

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Mfr.No.

CSB-E08809m

Alternative Names

hepatitis A virus cellular receptor 1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1, T cell immunoglobin domain and mucin domain protein 1, kidney injury molecule 1

Reactivity

Mouse

Detection Range

0.9 ng/mL-60 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Urine

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse A?1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse A?1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-99
1:2	Average %	95
	Range %	85-105
1:4	Average %	96
	Range %	90-105
1:8	Average %	93
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.490	2.451	2.471	2.337
500	2.049	1.985	2.017	1.883
250	1.357	1.385	1.371	1.237
125	0.866	0.837	0.852	0.718
62.5	0.517	0.524	0.521	0.387
31.2	0.347	0.355	0.351	0.217
15.6	0.244	0.228	0.236	0.102
0	0.135	0.132	0.134	?

Species

Mus musculus

Sensitivity

0.225 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q5QNS5
Antigen	hepatitis A virus cellular receptor 1

Applications

ELISA

Research Area

HAVCR1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Telluric Acid Ameliorates Endotoxemic Kidney Injury in Mice: Involvement of TLR4, Nrf2, and PI3K/Akt Signaling Pathways Mohamed AF.et al,Inflammation,Jul 6

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.