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Home / Primary Antibodies / IHC Antibodies

Phospho-ITCH (Tyr420) Antibody

SKU : BHA10560210

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA180347
Alternative Names	AIF4; AIP4; NAPP1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain.Chen X., Genomics 73:238-241(2001).Deloukas P., Nature 414:865-871(2001).Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH .
Involvement In Disease	Autoimmune disease, multisystem, with facial dysmorphism (ADMFD)
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
Subcellular Location	Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, Nucleus, Early endosome membrane, Peripheral membrane protein, Cytoplasmic side, Endosome membrane, Peripheral membrane protein, Cytoplasmic side

Uniprot	Q96J02
Gene Id	83737
Database Links	HGNC:13890 OMIM:606409 KEGG:hsa:83737 STRING:9606.ENSP00000363998 UniGene:Hs.632272
Function	Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Pathway	TNF signaling pathway Ubiquitin mediated proteolysis Endocytosis
Tissue Specificity	Widely expressed.
Weight	86,498 Da

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, IHC

Description

ELISA ; IHC, IHC ; ELISA

Dilution

IHC	1:50-1:100

Tested Application

ELISA,IHC;IHC:1:50-1:100

Datasheet
ELISA Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560210	CSB-PA180347	100ul	$360		Add to Cart

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