C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.Rapha l M篓顩歩vier, Mol. Endocrinol., Nov 2001; 15: 1953.Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α.
Involvement In Disease
Leukemia, acute myelogenous (AML)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.Rapha?l M?vier, Mol. Endocrinol., Nov 2001; 15: 1953.Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.
Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes
Protein Families
BZIP family, C/EBP subfamily
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.