Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418. Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949. R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953. Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418. Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949. R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953. Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
Tissue Specificity
Brain and testis.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.