Human Serine/threonine-protein kinase Sgk1(SGK1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL021189HU

Alternative Names

serum/glucocorticoid regulated kinase 1, RP1-188K17.1, SGK, serine/threonine protein kinase SGK

Reactivity

Human

Detection Range

23.44 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of sheep IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	85
	Range %	82-89
1:8	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

5.86 pg/mL

Limit Of Detection

450 nm

UniProt ID	O00141
Antigen	serum/glucocorticoid regulated kinase 1

Applications

ELISA

Research Area

SGK1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504294	CSB-EL021189HU	96T	$695	Add to Cart
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