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Home / ELISA Kit

Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit

SKU : BHE13703334

PRP6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.

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Mfr.No.	KTE61118
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF6.
Alternative Names	PRPF6; ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; p102 U5 small nuclea
Detection Method	Colorimetric
Kit Components	Human Pre-mRNA-processing factor 6 microplate Human Pre-mRNA-processing factor 6 standard Human Pre-mRNA-processing factor 6 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRPF6. No significant cross-reactivity or interference between Human PRPF6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PRP6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.
Notes	This Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF6 in samples. An antibody specific for PRPF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	24148

Applications	ELISA
Applications Notes	This Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF6 in samples. An antibody specific for PRPF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703334	KTE61118	48 T	$339		Add to Cart
BHE13703334	KTE61118	96 T	$589		Add to Cart
BHE13703334	KTE61118	96 Tx5	$2629		Add to Cart
BHE13703334	KTE61118	96 Tx50	$24569		Add to Cart

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