Human Nlr Family - Card Domain Containing 4

Mfr.No.

E3582Hu

Sensitivity

5.15ng/L

Detection Range

10-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human NLR Family, CARD Domain Containing 4 (also known as NLRC4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CARD, LRR, and NACHT-containing protein;Caspase recruitment domain-containing protein 12;Clan protein;Ice protease-activating factor;Ipaf;NLR family CARD domain-containing protein 4;NLRC 4;NLRC4

Molecular Weight

116159

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3582Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	828.9	47.21	5.7
Intra-Assay	2	18	1316.7	38.83	2.9
Intra-Assay	3	18	245.4	16.94	6.9

Background

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed: 15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

Protocol

These standard curves of E3582Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.675	2.667	2.613
	2.658
1000ng/L	1.507	1.437	1.383
	1.366
500ng/L	0.82	0.777	0.723
	0.733
250ng/L	0.493	0.446	0.392
	0.399
125ng/L	0.285	0.276	0.222
	0.266
0ng/L	0.045	0.054	0
	0.062

Gene ID	58484
UniProt ID	Q9NPP4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105061	E3582Hu	48T	$320		Add to Cart
BHE12105061	E3582Hu	96T	$458		Add to Cart

Human Nlr Family - Card Domain Containing 4 - NLRC4 ELISA Kit

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