| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Ultra-Pure Phi29 DNA Polymerase is a highly processive enzyme known for its powerful strand-displacement activity, making it ideal for isothermal amplification of both circular and linear DNA templates. It is particularly effective in techniques such as Rolling Circle Amplification (RCA), Multiple Displacement Amplification (MDA), and Whole Genome Amplification (WGA).
Thanks to its 3'→5' exonuclease activity, Ultra-Pure Phi29 DNA Polymerase boasts exceptional fidelity, enabling the amplification of even very small quantities of DNA templates. The enzyme undergoes an advanced, multi-step purification process that utilizes a combination of physical, chemical, and enzymatic methods to ensure the complete removal of contaminating genomic DNA. Rigorous quality control is performed, including a non-specific DNase Activity Assay and a qPCR DNA Contamination Test using a TaqMan probe, ensuring superior purity and performance.
| Product Component | Quantity |
|---|---|
| Ultra-Pure Phi29 DNA Polymerase | 100 μl (200 rxn) |
| 5X Ultra-Pure Phi29 DNA Polymerase Reaction Buffer1 | 500 μl |
1Buffer contains 0.5 μg/μl BSA. Keep at -20°C at all times except when using the product.
| Specification | Value |
|---|---|
| Enzyme Type | DNA Polymerase |
| Format | Enzyme Only |
| 3'→5' Proofreading | Yes |
| Storage Conditions | Store all components at -20°C. |
Phi29 DNA Polymerase has exceptional strand-displacement activity and processivity (>70 kb per binding event), enabling whole genome amplification and Rolling Circle Amplification (RCA) at 30°C without thermal cycling.
Yes. Phi29 DNA Polymerase has strong 3'→5' exonuclease proofreading activity, making it one of the highest-fidelity polymerases available with an error rate approximately 1,000-fold lower than Taq DNA polymerase.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Schimke KD; Vollmers C. Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and Chopper. PloS one. 2026;21:e0345168. DOI
- Hao X; Li Y; Hu X et al.. Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China. Frontiers in veterinary science. 2021;8:694089. DOI
- Oikonomopoulos S; Bayega A; Fahiminiya S et al.. Methodologies for Transcript Profiling Using Long-Read Technologies. Frontiers in genetics. 2020;11:606. DOI
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