Rat S100A11(S100 Calcium Binding Protein A11) CLIA Kit

Mfr.No.

E-CL-R0590

Antigen

S100A11

Alternative Names

MLN70 ; S100C ; Calgizzarin

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
20	32270	35694	33982	33950
10	11614	13326	12470	12438
5	5359	5141	5250	5218
2.5	2443	2605	2524	2492
1.25	1477	1289	1383	1351
0.625	886	848	867	835
0.31	618	628	623	591
0	32	32	32	--

Reactivity

Rat

Specificity

This kit recognizes Rat S100A11 in samples. No significant cross-reactivity or interference between Rat S100A11 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.313--20 ng/mL

Linearity

Samples were spiked with high concentrations of Rat S100A11 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-117	94-105	85-98
1:2	Average (%)	109	100	92
1:4	Range (%)	93-107	95-109	101-118
1:4	Average (%)	101	101	109
1:8	Range (%)	93-107	102-115	86-100
1:8	Average (%)	100	108	91
1:16	Range (%)	92-106	99-114	88-103
1:16	Average (%)	98	105	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat S100A11 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat S100A11 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.11	2.11	6.84	1.18	1.98	7.41
Standard deviation	0.12	0.22	0.42	0.15	0.20	0.45
C V (%)	10.81	10.43	6.14	12.71	10.10	6.07

Recovery

The recovery of Rat S100A11 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-114	105
EDTA plasma (n=5)	93-108	100
Cell culture media (n=5)	90-105	96

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Uniprot	Q6B345

Manual

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SKU	Supplier No.	Size	Your price
BHE10302387	E-CL-R0590	96T	$682	Add to Cart
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Rat S100A11(S100 Calcium Binding Protein A11) CLIA Kit

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