Rat MMP-12(MatriX Metalloproteinase 12) CLIA Kit

Mfr.No.

E-CL-R0034

Antigen

MMP-12

Alternative Names

MMP12 ; HME ; ME ; MME ; Macrophage Metalloelastase ; Macrophage Elastase

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	49995	57355	53675	53650
500	22203	22775	22489	22464
250	10470	9952	10211	10186
125	4666	5136	4901	4876
62.5	2463	2443	2453	2428
31.25	1338	1224	1281	1256
15.63	705	711	708	683
0	24	26	25	--

Specificity

This kit recognizes Rat MMP-12 in samples. No significant cross-reactivity or interference between Rat MMP-12 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Rat MMP-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-116	95-110	91-106
1:2	Average (%)	109	102	98
1:4	Range (%)	88-103	96-111	97-112
1:4	Average (%)	94	104	103
1:8	Range (%)	94-108	99-112	95-107
1:8	Average (%)	100	105	102
1:16	Range (%)	87-98	90-101	92-109
1:16	Average (%)	93	96	99

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-12 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	52.03	116.65	411.82	47.83	125.05	437.47
Standard deviation	6.56	11.83	46.49	4.11	8.89	32.46
C V (%)	12.61	10.14	11.29	8.59	7.11	7.42

Recovery

The recovery of Rat MMP-12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	98-117	106
EDTA plasma (n=5)	102-115	107
Cell culture media (n=5)	88-101	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	Q63341

Manual

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SKU	Supplier No.	Size	Your price
BHE10301913	E-CL-R0034	96T	$682	Add to Cart
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Rat MMP-12(Matrix Metalloproteinase 12) CLIA Kit

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