Rat IGFBP5(Insulin Like Growth Factor Binding Protein 5) CLIA Kit

Mfr.No.

E-CL-R0364

Antigen

IGFBP5

Alternative Names

IBP5

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	50512	56838	53675	53650
500	22036	22942	22489	22464
250	11120	9302	10211	10186
125	4831	4971	4901	4876
62.5	2529	2377	2453	2428
31.25	1306	1256	1281	1256
15.63	675	741	708	683
0	24	26	25	--

Reactivity

Rat

Specificity

This kit recognizes Rat IGFBP5 in samples. No significant cross-reactivity or interference between Rat IGFBP5 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Rat IGFBP5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-111	97-110	93-107
1:2	Average (%)	102	104	100
1:4	Range (%)	97-108	91-105	89-105
1:4	Average (%)	102	98	96
1:8	Range (%)	100-116	98-113	103-115
1:8	Average (%)	108	104	109
1:16	Range (%)	100-116	94-108	85-98
1:16	Average (%)	106	99	90

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IGFBP5 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IGFBP5 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	55.06	151.37	432.91	55.59	166.16	450.67
Standard deviation	6.61	13.15	26.84	7.20	13.11	29.61
C V (%)	12.01	8.69	6.20	12.95	7.89	6.57

Recovery

The recovery of Rat IGFBP5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	95-108	101
EDTA plasma (n=5)	91-108	98
Cell culture media (n=5)	99-114	105

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10302196	E-CL-R0364	96T	$682	Add to Cart
BHE10302196	E-CL-R0364	96T*5	$3069	Add to Cart
BHE10302196	E-CL-R0364	96T*10	$5797	Add to Cart
BHE10302196	E-CL-R0364	48T	$545.60	Add to Cart

Rat IGFBP5(Insulin Like Growth Factor Binding Protein 5) CLIA Kit

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