Rat GRbeta(Glucocorticoid Receptor beta) CLIA Kit

Mfr.No.

E-CL-R0281

Antigen

GRβ

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
500	47517	55557	51537	51508
250	19502	22494	20998	20969
125	9442	9350	9396	9367
62.5	4157	4867	4512	4483
31.25	2381	2217	2299	2270
15.625	1331	1169	1250	1221
7.81	695	785	740	711
0	28	30	29	--

Specificity

This kit recognizes Rat GRβ in samples. No significant cross-reactivity or interference between Rat GRβ and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

7.813--500 pg/mL

Linearity

Samples were spiked with high concentrations of Rat GRβ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	91-104	92-102	93-105
1:2	Average (%)	97	97	99
1:4	Range (%)	89-104	96-109	100-118
1:4	Average (%)	95	103	108
1:8	Range (%)	98-112	97-108	100-115
1:8	Average (%)	104	102	106
1:16	Range (%)	91-104	94-106	89-101
1:16	Average (%)	98	99	95

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GRβ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GRβ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	26.29	74.49	221.97	23.67	69.17	222.86
Standard deviation	2.10	5.26	14.10	2.50	5.75	20.86
C V (%)	7.99	7.06	6.35	10.56	8.31	9.36

Recovery

The recovery of Rat GRβ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-104	99
EDTA plasma (n=5)	93-109	100
Cell culture media (n=5)	90-102	96

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

4.69 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10302125	E-CL-R0281	96T	$682	Add to Cart
BHE10302125	E-CL-R0281	96T*5	$3069	Add to Cart
BHE10302125	E-CL-R0281	96T*10	$5797	Add to Cart
BHE10302125	E-CL-R0281	48T	$545.60	Add to Cart

Rat GRbeta(Glucocorticoid Receptor beta) CLIA Kit

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