Rat CXCR3(CXC-Chemokine Receptor 3) CLIA Kit

Mfr.No.

E-CL-R0190

Antigen

CXCR3

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	51020	60422	55721	55693
1000	22160	25788	23974	23946
500	11862	10202	11032	11004
250	5093	5495	5294	5266
125	2698	2518	2608	2580
62.5	1378	1244	1311	1283
31.25	665	683	674	646
0	27	29	28	--

Specificity

This kit recognizes Rat CXCR3 in samples. No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Rat CXCR3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	87-102	97-110	93-108
1:2	Average (%)	94	102	99
1:4	Range (%)	94-108	92-103	84-97
1:4	Average (%)	101	97	91
1:8	Range (%)	103-118	100-118	98-113
1:8	Average (%)	108	107	106
1:16	Range (%)	100-116	90-102	85-100
1:16	Average (%)	106	95	92

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CXCR3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CXCR3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	95.08	278.50	930.30	102.10	277.96	944.41
Standard deviation	10.08	21.89	84.29	11.61	29.44	103.41
C V (%)	10.60	7.86	9.06	11.37	10.59	10.95

Recovery

The recovery of Rat CXCR3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	90-102	97
EDTA plasma (n=5)	87-98	92
Cell culture media (n=5)	95-112	102

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	Q9JII9

Manual

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SKU	Supplier No.	Size	Your price
BHE10302046	E-CL-R0190	96T	$682	Add to Cart
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Rat CXCR3(CXC-Chemokine Receptor 3) CLIA Kit

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