Rat CTACK(Cutaneous T-Cell Attracting Chemokine) CLIA Kit

Mfr.No.

E-CL-R0189

Antigen

CTACK

Alternative Names

CCL27 ; SCYA27 ; ALP ; ILC ; Skinkine ; ESkine ; PESKY ; CTAK

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	49894	57456	53675	53650
500	20800	24178	22489	22464
250	10834	9588	10211	10186
125	4597	5205	4901	4876
62.5	2548	2358	2453	2428
31.25	1331	1231	1281	1256
15.63	696	720	708	683
0	25	25	25	--

Reactivity

Rat

Specificity

This kit recognizes Rat CTACK in samples. No significant cross-reactivity or interference between Rat CTACK and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Rat CTACK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	91-103	98-111	101-117
1:2	Average (%)	98	104	107
1:4	Range (%)	92-104	103-120	86-99
1:4	Average (%)	99	110	92
1:8	Range (%)	102-118	90-103	86-100
1:8	Average (%)	110	97	93
1:16	Range (%)	86-100	95-107	84-100
1:16	Average (%)	92	101	91

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CTACK were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CTACK were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	46.41	148.41	416.08	47.18	159.27	390.67
Standard deviation	5.69	15.94	42.65	4.29	18.32	39.15
C V (%)	12.26	10.74	10.25	9.09	11.50	10.02

Recovery

The recovery of Rat CTACK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	96-111	101
EDTA plasma (n=5)	98-113	104
Cell culture media (n=5)	84-99	91

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	D4AAL6

Manual

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SKU	Supplier No.	Size	Your price
BHE10302045	E-CL-R0189	96T	$682	Add to Cart
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BHE10302045	E-CL-R0189	48T	$545.60	Add to Cart

Rat CTACK(Cutaneous T-Cell Attracting Chemokine) CLIA Kit

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