Rat C-P(C-peptide) CLIA Kit

Mfr.No.

E-CL-R0028

Antigen

C-P

Alternative Names

Connecting Peptide

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
6000	64550	66716	65633	65614
3000	32717	37261	34989	34970
1500	17755	17753	17754	17735
750	8186	9128	8657	8638
375	4076	3902	3989	3970
187.5	1631	1619	1625	1606
93.75	424	448	436	417
0	18	20	19	--

Specificity

This kit recognizes Rat C-P in samples. No significant cross-reactivity or interference between Rat C-P and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

93.75--6000 pg/mL

Linearity

Samples were spiked with high concentrations of Rat C-P and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-110	91-104	86-98
1:2	Average (%)	100	96	91
1:4	Range (%)	91-102	85-97	102-116
1:4	Average (%)	97	91	110
1:8	Range (%)	100-116	92-107	87-100
1:8	Average (%)	108	100	93
1:16	Range (%)	85-97	86-97	98-112
1:16	Average (%)	90	91	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat C-P were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat C-P were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	304.20	613.60	2112.25	302.40	653.81	2238.05
Standard deviation	32.70	48.84	168.35	28.94	77.54	175.91
C V (%)	10.75	7.96	7.97	9.57	11.86	7.86

Recovery

The recovery of Rat C-P spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-108	101
EDTA plasma (n=5)	99-112	104
Cell culture media (n=5)	85-95	90

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

56.25 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301911	E-CL-R0028	96T	$682	Add to Cart
BHE10301911	E-CL-R0028	96T*5	$3069	Add to Cart
BHE10301911	E-CL-R0028	96T*10	$5797	Add to Cart
BHE10301911	E-CL-R0028	48T	$545.60	Add to Cart

Rat C-P(C-peptide) CLIA Kit

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