Rat BMG/beta2-MG(Beta-2-Microglobulin) CLIA Kit

Mfr.No.

E-CL-R0699

Antigen

BMG/β2-MG

Alternative Names

B2MG ; B2M

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
4000	55672	62490	59081	59046
2000	26884	28834	27859	27824
1000	13675	13293	13484	13449
500	6521	6689	6605	6570
250	3245	3241	3243	3208
125	1663	1499	1581	1546
62.50	709	801	755	720
0	35	35	35	--

Specificity

This kit recognizes Rat BMG/β2-MG in samples. No significant cross-reactivity or interference between Rat BMG/β2-MG and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

62.5--4000pg/mL

Linearity

Samples were spiked with high concentrations of Rat BMG/β2-MG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	94-108	91-104	97-113
1:2	Average (%)	102	98	103
1:4	Range (%)	100-116	103-115	92-106
1:4	Average (%)	106	109	99
1:8	Range (%)	100-114	91-104	90-102
1:8	Average (%)	106	96	96
1:16	Range (%)	99-117	101-116	90-100
1:16	Average (%)	107	110	95

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BMG/β2-MG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BMG/β2-MG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	193.34	606.37	1546.42	199.98	660.41	1680.76
Standard deviation	20.59	46.57	162.68	24.52	70.47	112.11
C V (%)	10.65	7.68	10.52	12.26	10.67	6.67

Recovery

The recovery of Rat BMG/β2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-108	100
EDTA plasma (n=5)	88-101	94
Cell culture media (n=5)	90-101	96

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

37.5 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10302482	E-CL-R0699	96T	$682	Add to Cart
BHE10302482	E-CL-R0699	96T*5	$3069	Add to Cart
BHE10302482	E-CL-R0699	96T*10	$5797	Add to Cart
BHE10302482	E-CL-R0699	48T	$545.60	Add to Cart

Rat BMG/beta2-MG(Beta-2-Microglobulin) CLIA Kit

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