Rat AVPR1A(Arginine Vasopressin Receptor 1A) CLIA Kit

Mfr.No.

E-CL-R0057

Antigen

AVPR1A

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
6000	62274	68992	65633	65614
3000	34737	35241	34989	34970
1500	19001	16507	17754	17735
750	8533	8781	8657	8638
375	4296	3682	3989	3970
187.5	1702	1548	1625	1606
93.75	434	438	436	417
0	19	19	19	--

Specificity

This kit recognizes Rat AVPR1A in samples. No significant cross-reactivity or interference between Rat AVPR1A and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

93.75--6000 ng/mL

Linearity

Samples were spiked with high concentrations of Rat AVPR1A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-115	98-112	96-110
1:2	Average (%)	108	105	101
1:4	Range (%)	99-112	99-115	102-120
1:4	Average (%)	105	106	109
1:8	Range (%)	94-108	97-114	101-114
1:8	Average (%)	101	105	108
1:16	Range (%)	93-107	94-108	98-114
1:16	Average (%)	98	101	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat AVPR1A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat AVPR1A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	333.06	672.13	2147.47	361.89	643.00	2277.62
Standard deviation	33.77	54.71	205.73	41.00	48.03	166.95
C V (%)	10.14	8.14	9.58	11.33	7.47	7.33

Recovery

The recovery of Rat AVPR1A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-99	94
EDTA plasma (n=5)	99-111	105
Cell culture media (n=5)	84-95	90

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

56.25 ng/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301933	E-CL-R0057	96T	$682	Add to Cart
BHE10301933	E-CL-R0057	96T*5	$3069	Add to Cart
BHE10301933	E-CL-R0057	96T*10	$5797	Add to Cart
BHE10301933	E-CL-R0057	48T	$545.60	Add to Cart

Rat AVPR1A(Arginine Vasopressin Receptor 1A) CLIA Kit

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