Rabbit SOD1(SuperoXide Dismutase 1, Soluble) ELISA Kit

Mfr.No.

E-EL-RB1695

Antigen

SOD1

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Rabbit SOD1 .During the reaction, Rabbit SOD1 in the sample or standard competes with a fixed amount of Rabbit SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit SOD1 Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rabbit SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	O.D	Average
10	0.337 0.387	0.362
5	0.459 0.491	0.475
2.5	0.676 0.656	0.666
1.25	0.9340000000000001 0.962	0.948
0.63	1.299 1.277	1.288
0.31	1.645 1.617	1.631
0.16	1.878 1.886	1.882
0	2.264 2.274	2.269

Specificity

This kit recognizes Rabbit SOD1 in samples. No significant cross-reactivity or interference between Rabbit SOD1 and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

0.156--10ng/mL

Linearity

Samples were spiked with high concentrations of Rabbit SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-100	91-106	91-106
1:2	Average (%)	91	97	98
1:4	Range (%)	81-94	93-103	95
1:4	Average (%)	87	98	95
1:8	Range (%)	84-97	89-104	94-109
1:8	Average (%)	90	96	99
1:16	Range (%)	87-100	90-103	89-103
1:16	Average (%)	93	96	96

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit SOD1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.54	1.05	4.14	0.58	1.03	3.79
Standard deviation	0.03	0.06	0.19	0.04	0.04	0.14
C V (%)	5.56	5.71	4.59	6.90	3.88	3.65

Recovery

The recovery of Rabbit SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	95-106	101
EDTA plasma (n=5)	85-99	92
Cell culture media (n=5)	89-105	96

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.09 ng/mL

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10307543	E-EL-RB1695	96T	$682	Add to Cart
BHE10307543	E-EL-RB1695	96T*5	$3069	Add to Cart
BHE10307543	E-EL-RB1695	96T*10	$5797	Add to Cart
BHE10307543	E-EL-RB1695	48T	$545.60	Add to Cart

Rabbit SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rabbit SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Rate it now..!!