Mouse TIMP-4(Tissue Inhibitors of Metalloproteinase 4) CLIA Kit

Mfr.No.

E-CL-M0654

Antigen

TIMP-4

Alternative Names

TIMP4

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	49401	57949	53675	53650
500	21649	23329	22489	22464
250	10699	9723	10211	10186
125	4852	4950	4901	4876
62.5	2559	2347	2453	2428
31.25	1344	1218	1281	1256
15.63	687	729	708	683
0	24	26	25	--

Reactivity

Mouse

Specificity

This kit recognizes Mouse TIMP-4 in samples. No significant cross-reactivity or interference between Mouse TIMP-4 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse TIMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	91-104	90-105	100-114
1:2	Average (%)	96	97	108
1:4	Range (%)	93-107	90-102	96-111
1:4	Average (%)	98	97	103
1:8	Range (%)	94-108	87-102	89-103
1:8	Average (%)	100	94	95
1:16	Range (%)	91-107	94-106	90-103
1:16	Average (%)	99	101	95

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TIMP-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TIMP-4 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	49.97	152.87	354.65	49.03	158.58	324.25
Standard deviation	5.24	12.43	39.69	5.70	16.35	29.99
C V (%)	10.49	8.13	11.19	11.63	10.31	9.25

Recovery

The recovery of Mouse TIMP-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-118	107
EDTA plasma (n=5)	96-111	102
Cell culture media (n=5)	88-102	93

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	Q9JHB3

Manual

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SKU	Supplier No.	Size	Your price
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Mouse TIMP-4(Tissue Inhibitors of Metalloproteinase 4) CLIA Kit

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