Mouse TIMP-3(Tissue Inhibitors of Metalloproteinase 3) CLIA Kit

Mfr.No.

E-CL-M0653

Antigen

TIMP-3

Alternative Names

TIMP3 ; SFD ; HSMRK222 ; K222 ; K222TA2

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	55317	56125	55721	55693
1000	22710	25238	23974	23946
500	11790	10274	11032	11004
250	4928	5660	5294	5266
125	2791	2425	2608	2580
62.5	1351	1271	1311	1283
31.25	632	716	674	646
0	28	28	28	--

Specificity

This kit recognizes Mouse TIMP-3 in samples. No significant cross-reactivity or interference between Mouse TIMP-3 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse TIMP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	87-100	91-101	94-110
1:2	Average (%)	92	96	101
1:4	Range (%)	93-105	83-98	96-108
1:4	Average (%)	99	90	102
1:8	Range (%)	99-115	93-105	89-101
1:8	Average (%)	105	99	95
1:16	Range (%)	91-106	92-104	98-116
1:16	Average (%)	98	97	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TIMP-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TIMP-3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	101.40	173.93	673.02	101.39	162.64	621.17
Standard deviation	12.79	13.27	58.69	11.41	19.34	45.16
C V (%)	12.61	7.63	8.72	11.25	11.89	7.27

Recovery

The recovery of Mouse TIMP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-108	100
EDTA plasma (n=5)	96-110	104
Cell culture media (n=5)	92-107	100

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P39876

Manual

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SKU	Supplier No.	Size	Your price
BHE10301837	E-CL-M0653	96T	$682	Add to Cart
BHE10301837	E-CL-M0653	96T*5	$3069	Add to Cart
BHE10301837	E-CL-M0653	96T*10	$5797	Add to Cart
BHE10301837	E-CL-M0653	48T	$545.60	Add to Cart

Mouse TIMP-3(Tissue Inhibitors of Metalloproteinase 3) CLIA Kit

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