Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) CLIA Kit

Mfr.No.

E-CL-M0518

Antigen

NT-proBNP

Alternative Names

NT-Pro-BNP

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
500	50281	52793	51537	51508
250	19601	22395	20998	20969
125	9790	9002	9396	9367
62.5	4225	4799	4512	4483
31.25	2302	2296	2299	2270
15.625	1335	1165	1250	1221
7.81	718	762	740	711
0	28	30	29	--

Specificity

This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

7.813--500 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	99-113	88-101	86-97
1:2	Average (%)	107	95	92
1:4	Range (%)	88-100	98-114	90-101
1:4	Average (%)	93	104	96
1:8	Range (%)	88-102	99-114	86-98
1:8	Average (%)	93	105	91
1:16	Range (%)	98-116	96-110	97-113
1:16	Average (%)	106	103	104

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NT-proBNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NT-proBNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	23.67	58.85	245.10	25.59	53.51	264.09
Standard deviation	2.45	6.99	16.32	2.88	6.19	25.27
C V (%)	10.35	11.88	6.66	11.25	11.57	9.57

Recovery

The recovery of Mouse NT-proBNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-109	101
EDTA plasma (n=5)	91-104	96
Cell culture media (n=5)	88-101	94

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

4.69 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301724	E-CL-M0518	96T	$682	Add to Cart
BHE10301724	E-CL-M0518	96T*5	$3069	Add to Cart
BHE10301724	E-CL-M0518	96T*10	$5797	Add to Cart
BHE10301724	E-CL-M0518	48T	$545.60	Add to Cart

Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) CLIA Kit

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