Mouse NGF(Nerve growth factor) CLIA Kit

Mfr.No.

E-CL-M0506

Antigen

NGF

Alternative Names

Beta-NGF ; HSAN5 ; NGFB ; NGF-B

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
800	48545	56739	52642	52616
400	21523	21945	21734	21708
200	9872	9544	9708	9682
100	4516	4588	4552	4526
50	2253	2123	2188	2162
25	1076	1044	1060	1034
12.50	495	523	509	483
0	25	27	26	--

Specificity

This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

12.5--800 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	103-115	88-102	99-114
1:2	Average (%)	109	93	105
1:4	Range (%)	87-100	98-115	84-96
1:4	Average (%)	93	106	91
1:8	Range (%)	98-113	85-100	93-109
1:8	Average (%)	104	92	100
1:16	Range (%)	99-114	102-118	90-104
1:16	Average (%)	107	109	96

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NGF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	40.05	96.94	395.79	41.08	88.94	369.96
Standard deviation	4.56	9.80	29.25	4.73	7.45	34.37
C V (%)	11.39	10.11	7.39	11.51	8.38	9.29

Recovery

The recovery of Mouse NGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	91-105	98
EDTA plasma (n=5)	87-100	94
Cell culture media (n=5)	92-104	98

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

7.5 pg/mL

Uniprot	P01139

Manual

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SKU	Supplier No.	Size	Your price
BHE10301715	E-CL-M0506	96T	$682	Add to Cart
BHE10301715	E-CL-M0506	96T*5	$3069	Add to Cart
BHE10301715	E-CL-M0506	96T*10	$5797	Add to Cart
BHE10301715	E-CL-M0506	48T	$545.60	Add to Cart

Mouse NGF(Nerve growth factor) CLIA Kit

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