Mouse IL-4(Interleukin 4) CLIA Kit

Mfr.No.

E-CL-M0041

Antigen

IL-4

Alternative Names

IL4 ; BCGF-1 ; BCGF1 ; BSF-1 ; BSF1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
400	47144	49274	48209	48172
200	18806	19840	19323	19286
100	9260	7852	8556	8519
50	3781	4401	4091	4054
25	2121	2055	2088	2051
12.5	1181	1107	1144	1107
6.25	683	691	687	650
0	36	38	37	--

Specificity

This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

6.25--400 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-109	97-110	86-102
1:2	Average (%)	103	104	93
1:4	Range (%)	97-112	91-101	98-114
1:4	Average (%)	105	96	107
1:8	Range (%)	85-97	91-104	84-96
1:8	Average (%)	91	98	91
1:16	Range (%)	91-103	97-113	99-115
1:16	Average (%)	97	103	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-4 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	21.44	57.26	142.71	22.34	59.53	129.61
Standard deviation	2.57	4.57	12.47	2.48	5.64	13.74
C V (%)	11.99	7.98	8.74	11.10	9.47	10.60

Recovery

The recovery of Mouse IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-114	105
EDTA plasma (n=5)	86-101	93
Cell culture media (n=5)	97-114	104

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

3.75 pg/mL

Uniprot	P07750

Manual

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SKU	Supplier No.	Size	Your price
BHE10301314	E-CL-M0041	96T	$682	Add to Cart
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Mouse IL-4(Interleukin 4) CLIA Kit

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