Mouse IgG2a(Immunoglobulin G2a) CLIA Kit

Mfr.No.

E-CL-M0429

Antigen

IgG2a

Alternative Names

IgG2a

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
100	37146	37962	37554	37521
50	15853	16629	16241	16208
25	7821	7263	7542	7509
12.5	3402	3962	3682	3649
6.25	1927	1823	1875	1842
3.125	1056	946	1001	968
1.56	548	596	572	539
0	31	35	33	--

Specificity

This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

1.563--100 ng/mL

Linearity

Samples were spiked with high concentrations of Mouse IgG2a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-110	88-101	90-103
1:2	Average (%)	102	94	97
1:4	Range (%)	103-116	101-119	92-104
1:4	Average (%)	109	108	97
1:8	Range (%)	87-103	93-108	88-101
1:8	Average (%)	94	99	94
1:16	Range (%)	92-104	96-111	94-107
1:16	Average (%)	97	104	101

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgG2a were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgG2a were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.58	14.98	41.52	4.55	14.38	40.61
Standard deviation	0.43	1.48	4.18	0.42	1.03	3.01
C V (%)	9.39	9.88	10.07	9.23	7.16	7.41

Recovery

The recovery of Mouse IgG2a spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	95-109	100
EDTA plasma (n=5)	91-105	96
Cell culture media (n=5)	101-115	106

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.94 ng/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301650	E-CL-M0429	96T	$682	Add to Cart
BHE10301650	E-CL-M0429	96T*5	$3069	Add to Cart
BHE10301650	E-CL-M0429	96T*10	$5797	Add to Cart
BHE10301650	E-CL-M0429	48T	$545.60	Add to Cart

Mouse IgG2a(Immunoglobulin G2a) CLIA Kit

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