Mouse I-TAC/CXCL11(Interferon Inducible T-Cell Alpha Chemoattractant) CLIA Kit

Mfr.No.

E-CL-M0052

Antigen

I-TAC

Alternative Names

CXCL11 ; H174 ; IP-9 ; IP9 ; SCYB11 ; SCYB9B ; b-R1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	52163	55187	53675	53650
500	21769	23209	22489	22464
250	10767	9655	10211	10186
125	4528	5274	4901	4876
62.5	2525	2381	2453	2428
31.25	1305	1257	1281	1256
15.63	663	753	708	683
0	25	25	25	--

Reactivity

Mouse

Specificity

This kit recognizes Mouse I-TAC in samples. No significant cross-reactivity or interference between Mouse I-TAC and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse I-TAC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-116	100-116	93-106
1:2	Average (%)	108	107	100
1:4	Range (%)	101-118	88-100	87-100
1:4	Average (%)	109	95	93
1:8	Range (%)	86-102	102-118	86-96
1:8	Average (%)	93	108	91
1:16	Range (%)	85-101	96-111	91-103
1:16	Average (%)	92	102	96

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse I-TAC were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse I-TAC were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.71	97.44	430.27	47.00	98.99	462.70
Standard deviation	5.67	11.34	35.71	5.12	7.48	53.07
C V (%)	10.96	11.64	8.30	10.89	7.56	11.47

Recovery

The recovery of Mouse I-TAC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	96-110	101
EDTA plasma (n=5)	100-117	108
Cell culture media (n=5)	92-108	99

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	Q9JHH5

Manual

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SKU	Supplier No.	Size	Your price
BHE10301323	E-CL-M0052	96T	$682	Add to Cart
BHE10301323	E-CL-M0052	96T*5	$3069	Add to Cart
BHE10301323	E-CL-M0052	96T*10	$5797	Add to Cart
BHE10301323	E-CL-M0052	48T	$545.60	Add to Cart

Mouse I-TAC/CXCL11(Interferon Inducible T-Cell Alpha Chemoattractant) CLIA Kit

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