Mouse HSP-60(Heat Shock Protein 60) CLIA Kit

Mfr.No.

E-CL-M0374

Antigen

HSP-60

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	50189	61253	55721	55693
1000	23944	24004	23974	23946
500	11431	10633	11032	11004
250	5100	5488	5294	5266
125	2761	2455	2608	2580
62.5	1407	1215	1311	1283
31.25	651	697	674	646
0	27	29	28	--

Specificity

This kit recognizes Mouse HSP-60 in samples. No significant cross-reactivity or interference between Mouse HSP-60 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse HSP-60 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-105	100-118	88-103
1:2	Average (%)	100	108	95
1:4	Range (%)	86-101	86-102	94-110
1:4	Average (%)	92	93	101
1:8	Range (%)	92-107	93-107	83-95
1:8	Average (%)	99	100	90
1:16	Range (%)	84-99	104-120	102-118
1:16	Average (%)	90	110	109

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HSP-60 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HSP-60 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	99.22	241.58	939.43	96.72	264.77	974.56
Standard deviation	12.27	21.98	57.59	12.40	31.35	78.26
C V (%)	12.37	9.10	6.13	12.82	11.84	8.03

Recovery

The recovery of Mouse HSP-60 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	90-104	97
EDTA plasma (n=5)	93-107	100
Cell culture media (n=5)	94-108	100

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P63038

Manual

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SKU	Supplier No.	Size	Your price
BHE10301598	E-CL-M0374	96T	$682	Add to Cart
BHE10301598	E-CL-M0374	96T*5	$3069	Add to Cart
BHE10301598	E-CL-M0374	96T*10	$5797	Add to Cart
BHE10301598	E-CL-M0374	48T	$545.60	Add to Cart

Mouse HSP-60(Heat Shock Protein 60) CLIA Kit

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