Mouse GAL2(Galectin 2) CLIA Kit

Mfr.No.

E-CL-M0318

Antigen

GAL2

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
500	48837	54237	51537	51508
250	19143	22853	20998	20969
125	10080	8712	9396	9367
62.5	4337	4687	4512	4483
31.25	2446	2152	2299	2270
15.625	1286	1214	1250	1221
7.81	729	751	740	711
0	28	30	29	--

Specificity

This kit recognizes Mouse GAL2 in samples. No significant cross-reactivity or interference between Mouse GAL2 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

7.813--500 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse GAL2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-97	91-103	101-115
1:2	Average (%)	92	97	106
1:4	Range (%)	91-104	97-112	85-99
1:4	Average (%)	97	105	92
1:8	Range (%)	92-106	100-118	97-109
1:8	Average (%)	98	108	103
1:16	Range (%)	90-103	98-118	90-104
1:16	Average (%)	96	107	97

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GAL2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GAL2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	24.21	70.15	241.21	24.20	73.14	249.42
Standard deviation	2.73	6.84	21.47	2.63	7.23	19.55
C V (%)	11.28	9.75	8.90	10.87	9.89	7.84

Recovery

The recovery of Mouse GAL2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	85-96	90
EDTA plasma (n=5)	90-104	96
Cell culture media (n=5)	88-104	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

4.69 pg/mL

Uniprot	Q9CQW5

Manual

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SKU	Supplier No.	Size	Your price
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Mouse GAL2(Galectin 2) CLIA Kit

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