Mouse D1R(Dopamine D1 receptor) CLIA Kit

Mfr.No.

E-CL-M0264

Antigen

D1R

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
5000	57694	64818	61256	61233
2500	32064	36644	34354	34331
1250	19757	16581	18169	18146
625	8663	10121	9392	9369
312.5	4877	4789	4833	4810
156.25	2599	2423	2511	2488
78.13	1291	1387	1339	1316
0	22	24	23	--

Reactivity

Mouse

Specificity

This kit recognizes Mouse D1R in samples. No significant cross-reactivity or interference between Mouse D1R and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

78.125--5000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse D1R and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-109	104-115	101-116
1:2	Average (%)	103	109	110
1:4	Range (%)	103-121	100-114	85-98
1:4	Average (%)	110	106	91
1:8	Range (%)	88-99	86-99	95-109
1:8	Average (%)	94	92	101
1:16	Range (%)	99-116	88-103	100-113
1:16	Average (%)	107	94	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse D1R were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse D1R were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	274.27	467.74	1954.85	285.73	510.37	1801.46
Standard deviation	26.38	39.06	194.51	31.69	49.15	115.47
C V (%)	9.62	8.35	9.95	11.09	9.63	6.41

Recovery

The recovery of Mouse D1R spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	85-96	91
EDTA plasma (n=5)	97-108	102
Cell culture media (n=5)	100-115	107

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

46.88 pg/mL

Uniprot	Q61616

Manual

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Mouse D1R(Dopamine D1 receptor) CLIA Kit

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