Mouse CCL1(Chemokine C-C-Motif Ligand 1) CLIA Kit

Mfr.No.

E-CL-M0185

Antigen

CCL1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	54460	56982	55721	55693
1000	22996	24952	23974	23946
500	11219	10845	11032	11004
250	4922	5666	5294	5266
125	2712	2504	2608	2580
62.5	1411	1211	1311	1283
31.25	671	677	674	646
0	27	29	28	--

Specificity

This kit recognizes Mouse CCL1 in samples. No significant cross-reactivity or interference between Mouse CCL1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse CCL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-108	91-105	86-100
1:2	Average (%)	99	96	92
1:4	Range (%)	97-108	97-113	90-103
1:4	Average (%)	103	104	95
1:8	Range (%)	89-103	96-111	90-103
1:8	Average (%)	95	103	97
1:16	Range (%)	91-106	85-99	88-102
1:16	Average (%)	97	90	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CCL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CCL1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	98.89	192.52	722.97	104.18	195.55	687.16
Standard deviation	9.10	15.25	79.45	8.72	16.00	49.82
C V (%)	9.20	7.92	10.99	8.37	8.18	7.25

Recovery

The recovery of Mouse CCL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	92-105	99
EDTA plasma (n=5)	101-116	107
Cell culture media (n=5)	89-104	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P10146

Manual

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SKU	Supplier No.	Size	Your price
BHE10301435	E-CL-M0185	96T	$682	Add to Cart
BHE10301435	E-CL-M0185	96T*5	$3069	Add to Cart
BHE10301435	E-CL-M0185	96T*10	$5797	Add to Cart
BHE10301435	E-CL-M0185	48T	$545.60	Add to Cart

Mouse CCL1(Chemokine C-C-Motif Ligand 1) CLIA Kit

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