Mouse CACNa1E(Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit) ELISA Kit

Mfr.No.

ELK6875

Antigen

CACNa1E

Alternative Names

BII; CACNL1A6; CACH6; Brain calcium channel II; Calcium channel, L type, alpha-1 polypeptide, isoform 6; Voltage-gated calcium channel subunit alpha Cav2.3

Reactivity

Mouse

Analytical Method

Sandwich

Assay Length

3.5h

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit(CACNa1E). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit(CACNa1E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit(CACNa1E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit(CACNa1E) in the samples is then determined by comparing the OD of the samples to the standard curve.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CACNa1E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-96%	86-98%	91-103%	87-90%
EDTA plasma(n=5)	78-92%	79-93%	94-106%	83-97%
Heparin plasma(n=5)	80-92%	86-102%	87-103%	85-101%

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)?CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CACNa1E and the recovery rates were calculated by comparing the measured value to the expected amount of CACNa1E in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	87-99%	93%

Sample Type

Sandwich

Sensitivity

0.125 ng/mL

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
20.00	2.119	2.013
10.00	1.616	1.510
5.00	1.283	1.177
2.50	0.948	0.842
1.25	0.509	0.403
0.63	0.326	0.220
0.32	0.235	0.129
0.00	0.106	0.000

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206300	ELK6875	96T	$458		Add to Cart
BHE15206300	ELK6875	48T	$320		Add to Cart

Mouse CACNa1E(Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit) ELISA Kit

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